Cette biodégradation est basée sur le fonctionnement d’un consortium bactérien complexe, composé de bactéries autotrophes et hétérotrophes, associées sous. Request PDF on ResearchGate | Influence possible des protozoaires sur le taux de mortalité des bactéries autotrophes nitrifiantes | Le modele de l’IAWQ du. Bactérie (Bacteria): Les bactéries sont des organismes vivants unicellulaires, très Les bactéries autotrophes telles que Nitrobacter, active dans les processus.

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Change the order of display of the official languages of Canada English first French first Option to display the non-official languages Spanish or Portuguese Neither Spanish Portuguese Display definitions, contexts, etc. By acting this way, inhibition of autotrophic C incorporation by nitrifying bacteria was also complete, while heterotrophic incorporation was unaffected.

Continuous enrichment cultures of nitrifying bacteria were obtained from an inoculum coming from the Seine estuary freshwater section.

Biovolumes were estimated according to cell size and autotrlphe in biomasses according to a conversion factor determined experimentally with a carbon analyser.

This potential activity can be used to estimate the nitrifying biomass by considering a conversion factor ahtotrophe 0. The method can also be used by placing the sample in optimal temperature, pH, oxygen and ammonium conditions for nitrifying bacteria, to allow potential nitrifying activity measurements.

The organic solvent enhanced heterotrophic incorporation of C which totally masked out the autotrophic contribution of nitrifying bacteria. For this reason N-serve was added in the empty flask before the sample to allow the evaporation of the solvent. Bicarbonate was measured by acid titration in natural water samples, and with the evolution method for culture samples.

This work presents less reassessment of the autotrophic nitrifying activity measurement by the 14C-bicarbonate incorporation method and its use to estimate the biomass of nitrifying bacteria.

To measure potential nitrifying activities, the optimal growth conditions of nitrifying bacteria were determined on enrichment cultures: Ammonium was measured with the indophenol blue method, nitrate was reduced in nitrite on a cadmium bed and nitrite was measured with the sulfanilamide method. The measurement of the activity and biomass of nitrifying bacteria is thus essential to understand and quantify the general nitrogen fluxes in those environments.


Revue des sciences de l’eau11 2— Different methods of measuring the nitrifying activity exist. This factor is considered to be constant in the standard incubation conditions.

This factors allowed us to establish a relationship between potential nitrifying activity measurements and nitrifying biomass: Access a collection of Canadian resources on all aspects of English and French, including quizzes. Writing tools A collection of writing tools that cover the many facets of English and French grammar, style and usage.

However, the ethanolic solution of N-serve had an unwanted result on C incorporation. The determined optimal growth rate was 0. Revue des sciences de l’eau 11, no. The general validity of the method was demonstrated by experiments on nitrification inhibitors in enrichment cultures. The main differences of our protocol to the original ones is that we propose the use of a combination of 2 inhibitors, N-serve and chlorate, and the elimination by evaporation of the organic solvent of N-serve ethanol to avoid any interference with the heterotrophic populations.

The inhibitors effects were also determined on the C incorporation rates of heterotrophic bacteria. The method can be used in in situ conditions, to allow real nitrifying activities measurements in samples. Our conclusion is that the results presented in this paper allow bactetie validation of the 14C-bicarbonate bacterje method with and without inhibitors to measure the nitrifying activity. Glossaries and vocabularies Access Translation Bureau glossaries and vocabularies. Classical culture techniques most probable number autofrophe immunofluorescence techniques are known autotropphe greatly underestimate the numbers of active organisms.

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N-serve, allyl thio-urea, acetylene, methylfluoride and dimethyl ether, as most used. In which subject field? Several methods were used for our study: These experiments consisted in measuring the effect of different combinations of N-serve, ethanol the organic solvent of N-serve and chlorate, on N-oxidation rates and C incorporation rates on samples of the two nitrifying enrichment cultures ammonium- and nitrite-oxidising bacteria.


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Une réévaluation de la methode d’incorporation – Revue des sciences de l’eau – Érudit

Environmental Studies and Analyses [1]. The first methods developed were based on the use of specific nitrification inhibitors: Download the article in PDF bactetie read it. The language you choose must correspond to the language of the term you have entered. In this case, carbon incorporation rates can be converted in ammonium oxidation rates with the use of the conversion factor 0. Bacteria which are able to multiply by using inorganic matter as the only source of carbon and nitrogen.

At the present time, the most used methods are dealing with isotopic tracers: By regenerating oxidised forms of nitrogen nitratethe nitrification auttorophe plays an important role in the nitrogen cycle of aquatic environments. Switch to classic view.

The most frequently used enumeration methods of nitrifying bacteria aautotrophe not very satisfactory. Recently developed gene-probes techniques work well for the identification of particular strains, but are not yet useful for the numeration.

A good alternative to these methods consists in the measurement of potential nitrifying activity which is correlated to the nitrifying biomass.

C incorporation rates are measured by the incubation of samples with 14C-bicarbonate, the samples being filtered on 0. Nitrifying cell numbers and size in the pure cultures were determined by epifluorescence with a microscope, after DAPI staining.

They consist in measuring differences of ammonium, nitrite and nitrate dynamics in an inhibited and control sample during time.